Mediator and RNA polymerase II clusters associate in transcription-dependent condensates.

WK Cho*, JH Spille*, M Hecht, C Lee, C Li, V Grube, II Cisse. Science, eaar4199 (2018).

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[Science Perspective] [Science News Article]

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates.

YE Guo, JC Manteiga, JE Henninger, BR Sabari, A Dall’Agnese, NM Hannett, JH Spille, LK Afeyan, AV Zamudio, K Shrinivas, BJ Abraham, A Boija, TM Decker, JK Rimel, CB Fant, TI Lee, II Cisse, PA Sharp, DJ Taatjes, RA Young . Nature 572, 543-548 (2019).

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[Trends in Biochemical Sciences Spotlight]

Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields.

AA Agbleke et al.. Mol Cell (2020).

[Link]

A CRISPR/Cas9 platform for MS2-labelling of single mRNA in live stem cells.

JH Spille, M Hecht, V Grube, W Cho, C Lee, II Cissé. Methods 153, 35-45 (2019).

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Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking.

JH Spille, TP Kaminski, K Scherer, JS Rinne, A Heckel, U Kubitscheck. Nucleic Acids Research 43 (2), e14 (2014).

[Link] [PDF] [Read more ...]

Igh Locus Polymorphism May Dictate Topological Chromatin Conformation and V Gene Usage in the Ig Repertoire

C Watson, A Kenter, and JH Spille, Frontiers in Immunology (2021).

Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields.

A Agbleke et al. Mol Cell 79(6), 881-901 (2020). [Link]

Mediator condensates localize signaling factors to key cell identity genes. AV Zamudio, A Dall’Agnese, JE Henninger, JC Manteiga, LK Afeyan, NM Hannett, EL Coffey, CL Li, O Oksuz, JBR Sabari, A Boija, IA Klein, SW Hawken, JH Spille, TM Decker, II Cisse, BJ Abraham, TI Lee, DJ Taatjes,J Schuijers, RA Young.

Mol Cell, 76 (5), 753-766. e6 (2019). [Link]

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates. YE Guo, JC Manteiga, JE Henninger, BR Sabari, A Dall’Agnese, NM Hannett, JH Spille, LK Afeyan, AV Zamudio, K Shrinivas, BJ Abraham, A Boija, TM Decker, JK Rimel, CB Fant, TI Lee, II Cisse, PA Sharp, DJ Taatjes, RA Young.

Nature 572, 543-548 (2019). [Link] [Read more ...]

[Trends in Biochemical Sciences Spotlight]

A first order phase transition mechanism underlies protein aggregation in mammalian cells. A Narayanan, A Meriin, JO Andrews, JH Spille, MY Sherman, II Cisse.

ELife 8, e39695 (2019). [Link][PDF]

A CRISPR/Cas9 platform for MS2-labelling of single mRNA in live stem cells. JH Spille, M Hecht, V Grube, W Cho, C Lee, II Cissé.

Methods 153, 35-45 (2019). [Link] [Read more ...]

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates. WK Cho*, JH Spille*, M Hecht, C Lee, C Li, V Grube, II Cisse.

Science, eaar4199 (2018). [Link] [Read more ...]

[Science Perspective] [Science News Article]

qSR: a quantitative super-resolution analysis tool reveals the cell-cycle dependent organization of RNA Polymerase I in live human cells. JO Andrews, W Conway, WK Cho, A Narayanan, JH Spille, N Jayanth, T Inoue, S Mullen, J Thaler, II Cissé.

Scientific Reports 8 (1), 7424 (2018). [Link]

Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB. A Badrinarayanan, TBK Le, JH Spille, II Cisse, MT Laub.

PLoS Genetics 13 (5), e1006783 (2017). [Link]

RNA Polymerase II cluster dynamics predict mRNA output in living cells. WK Cho, N Jayanth, BP English, T Inoue, JO Andrews, W Conway, JB Grimm, JH Spille, LD Lavis, T Lionnet, II Cisse.

Elife 5, e13617 (2016). [Link] [PDF] [Read more ...]

Labelling and imaging of single endogenous messenger RNA particles in vivo. JH Spille, U Kubitscheck.

J Cell Sci 128 (20), 3695-3706 (2015). [Link] [PDF]

The lantibiotic nisin induces lipid II aggregation, causing membrane instability and vesicle budding. KM Scherer, JH Spille, HG Sahl, F Grein, U Kubitscheck.

Biophysical Journal 108 (5), 1114-1124 (2015). [Link]

Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking. JH Spille, TP Kaminski, K Scherer, JS Rinne, A Heckel, U Kubitscheck.

Nucleic Acids Research 43 (2), e14 (2014). [Link] [PDF] [Read more ...]

Reelin and CXCL12 regulate distinct migratory behaviors during the development of the dopaminergic system. GO Bodea, JH Spille, P Abe, AS Andersson, A Acker-Palmer, R Stumm, U Kubitscheck, S Blaess.

Development 141 (3), 661-673 (2014). [Link]

Cutting Edge: The UNC93B1 Tyrosine-Based Motif Regulates Trafficking and TLR Responses via Separate Mechanisms. K Pelka, K Phulphagar, J Zimmermann, R Stahl, JL Schmid-Burgk, T Schmidt, JH Spille, LI Labzin, S Agrawal, ER Kandimalla, JL Casanova, V Hornung, A Marshak-Rothstein, S Höning, E Latz.

The Journal of Immunology 193 (7), 3257-3261 (2014). [Link] [PDF]

Nuclear Trafficking and Export of Single, Native mRNPs in Chironomus tentans Salivary Gland Cells. TP Kaminski, JH Spille, C Nietzel, JP Siebrasse, U Kubitscheck.

Methods in Molecular Biology: Imaging Gene Expression, 73-85 (2013). [Link]

Dynamic three-dimensional tracking of single fluorescent nanoparticles deep inside living tissue. JH Spille, T Kaminski, HP Königshoven, U Kubitscheck.

Optics Express 20 (18), 19697-19707 (2012). [Link] [PDF] [Read more ...]

A cylindrical zoom lens unit for adjustable optical sectioning in light sheet microscopy. JG Ritter, JH Spille, T Kaminski, U Kubitscheck.

Biomedical Optics Express 2 (1), 185-193 (2011). [Link] [PDF]

Rotational Diffusion of the α2a Adrenergic Receptor Revealed by FlAsH Labeling in Living Cells. JH Spille, A Zürn, C Hoffmann, MJ Lohse, GS Harms.

Biophysical Journal 100 (4), 1139-1148 (2011).

[Link]